Journal: Neuro-Oncology

Article Title: Functional role of endothelial adhesion molecules in the early stages of brain metastasis

doi: 10.1093/neuonc/not222

Figure Lengend Snippet: Colocalization of adhesion molecules with endothelial cells. Triple-color fluorescence images showing colocalization (arrows) between tumor cells and endothelial marker von Willebrand Factor (vWF) in the 4T1-GFP model. All tumor cells injected were GFP positive, and therefore the green fluorescence corresponds to tumor cells. An AMCA fluorophore was used to localize the endothelial markers, and hence the blue color reflects vWF. To detect the 12 cellular adhesion molecules, a Cy3 fluorophore was used (red). Scale bar 50 µm. High magnification insets show regions of colocalization (indicated by arrows).

Article Snippet: The following secondary antibodies were used as appropriate: Cy3-streptavidin conjugate (1:200;Invitrogen); anti-rabbit AMCA-conjugated (1:100; Vector Laboratories); biotinylated polyclonal antibody to chicken raised in rabbit (Abcam); biotinylated polyclonal antibody to goat raised in horse (Vector Laboratories); polyclonal antibody to rat raised in rabbit (Vector Laboratories); and biotinylated polyclonal to rabbit raised in goat (Vector Laboratories).

Techniques: Fluorescence, Marker, Injection

Journal: The Journal of Neuroscience

Article Title: Clustered Fine Compartmentalization of the Mouse Embryonic Cerebellar Cortex and Its Rearrangement into the Postnatal Striped Configuration

doi: 10.1523/JNEUROSCI.1710-12.2012

Figure Lengend Snippet: Antibody specification

Article Snippet: After immunohistochemistry, some sections were fluorescently counterstained with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI; -cellstain-DAPI, D212, Dojindo). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antigen Immunogen Manufacturer, species, mono- or polyclonal, catalog and lot no Dilution used Primary antibodies Calbindin-D28k Calbindin-D28k purified from chicken gut was used as the immunogen Sigma-Aldrich, mouse monoclonal, Cat. # C-8666, Lot # 058H4812 1:6000 Calbindin-D28k Synthetic peptide derived from amino acids 185-199 of human Calbindin-D-28K AnaSpec, rabbit polyclonal, Cat. # 53283, Lot # GL141 1:4000 EphA4 Purified, NS0-derived, recombinant mouse EphA4 extracellular domain R&D Systems, goat polyclonal, Cat. # AF641, Lot # BVX0308091 1:1000 FoxP2 Synthetic peptide derived from C-terminal amino acids of isoforms I, II, IV, and V of human FOXP2: REIEEEPLSEDLE Everest Biotech, goat polyclonal, Cat. # EB05226, Lot # 160409 1:5000 for fluorescent, 1:10000 for DAB FoxP2 A KLH-conjugated synthetic peptide between 664 and 693 amino acids from the C-terminal region of human FOXP2 Abgent, rabbit polyclonal, Cat. # AP5753b, Lot # SA100916AA 1:1000 Pcdh10 The cytoplasmic domain of the OL-pc isoform ( Aoki et al., 2003 ) Millipore, rat monoclonal, clone 5G10, Cat. # MABT20, Lot # NRG1759424 1:1600 PLCβ4 Amino acids 876-1115 of PLCβ4 of human origin Santa Cruz Biotechnology, rabbit polyclonal, Cat. #sc-20760, Lot # A1508 1:150 RORα Amino acids 196-260 mapping within an internal region of RORα2 of human origin Santa Cruz Biotechnology, rabbit polyclonal, Cat. # sc-28612, Lot # I0909 1:100 Secondary antibodies Anti-goat IgG, biotin-conjugated Vector Laboratories, rabbit, Cat. # BA-5000 1:333 Anti-rabbit IgG, biotin-conjugated Vector Laboratories, goat, Cat. # BA-1000 1:333 Anti-mouse IgG, biotin-conjugated Vector Laboratories, goat, Cat. # BA-9200 1:333 Anti-goat IgG, fluorescein-conjugated Jackson ImmunoResearch, donkey, Cat. # 705-095-147 1:333 Anti-goat IgG, DyLight 488-conjugated Jackson ImmunoResearch, donkey, Cat. # 705-485-147 1:400 Anti-rabbit IgG, Texas Red-conjugated Jackson ImmunoResearch, donkey, Cat. # 711-075-152 1:333 Anti-rat IgG, DyLight 594-conjugated Jackson ImmunoResearch, donkey, Cat. # 712-515-153 1:333 Open in a separate window The data are based on manufacturer's data sheets and in some cases on communications with manufacturers.

Techniques: Purification, Derivative Assay, Recombinant, Plasmid Preparation